概要
エピジェネティクス分野で最先端を進んできたZymoResearch社の次世代シークエンシングを用いたエピジェネティクス解析受託サービスです。
＊ 任意の遺伝子配列に対してバイサルファイトシークエンシングを行う受託サービス
「MethylCheck Targeted Sequencing for DNA Methylation Analysis」については、
こちらをご覧ください。
＊各種メチル化解析受託サービス、創薬支援サービスをまとめてご紹介したエピジェネティクスカタログ＜受託サービス＞ページについては
こちらをご覧ください。
特長
- Whole Genome解析
全ゲノムにおけるメチル化状態（Methylome）を把握できる唯一のサービス（Methyl-MaxiSeq）
- Genome Wide解析
・DNAメチル化（5-mC）を捉えるClassic RRBS解析、およびCoverageを上げたMethyl-MiniSeqサービスを用意
- Methyl-Mini Seq解析の測定カバー率（fig.1）
| 個数 | 網羅率（％） |
| CpG部位 | ～1,500,000 | － |
| 遺伝子のプロモーター | ＞18,400 | ≧75 |
| CpGアイランド | ＞22,950 | ≧80 |
| Browser Tracks |
Result Tables |
Hexbin Plot |
Genomic Region Pie Chart |
Clustering Heatmap |
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| Region Coverage Pie Chart |
Methylation Level Boxplot |
Methylation Overview |
Read Coverage Plot |
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参考文献
- Barua S, et al. (2014) Single-base resolution of mouse offspring brain methylome reveals epigenome modifications caused by gestational folic acid. Epigenetics Chromatin. 7(1):3. doi: 10.1186/1756-8935-7-3.
- Diede SJ, et al. (2013) Fundamental differences in promoter CpG island DNA hypermethylation between human cancer and genetically engineered mouse models of cancer. Epigenetics. 8(12): 1254-1260.
- MacQuarrie KL, et al. (2013) Comparison of genome-wide binding of MyoD in normal human myogenic cells and rhabdomyosarcomas identifies regional and local suppression of promyogenic transcription factors. Mol Cell Biol. 33(4):773-84.
- Vicente-Dueñas C, et al. (2012) A novel molecular mechanism involved in multiple myeloma development revealed by targeting MafB to haematopoietic progenitors. EMBO J. 31(18):3704-17.
サービスの種類
|
5-mC |
| Genome Wide |
Whole Genome |
| Classic RRBS |
Methyl-MiniSeq |
Methyl-MaxiSeq |
| Capable with low DNA input? |
Yes |
Yes |
Yes |
| Single-base Resolution? |
Yes |
Yes |
Yes |
| Methylome Coverage＊ |
1.5～2million sites |
3～4million sites |
Entire methylome |
| Quantitative Analysis? |
Yes |
Yes |
Yes |
| Genomic Regions covered |
Nearly all CpG islands and gene promoters |
Twice as many unique CpG sites compared to Classic RRBS |
Entire methylome |
| Notes |
Efficient genome-wide analysis |
Robust biomarker discovery |
Complete methylation analysis |
＊calculation based on human genome
＊depends on capture efficiency and methylation levels
